ClickIn: a flexible protocol for quantifying mitochondrial uptake of nucleobase derivatives
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چکیده
منابع مشابه
ClickIn: a flexible protocol for quantifying mitochondrial uptake of nucleobase derivatives
There is an increasing interest in targeting molecules to the mitochondrial matrix. Many proteins are naturally imported through the translocase complexes found in the outer and inner mitochondrial membranes. One possible means for importing molecules is therefore to use a mitochondrial pre-protein as a vector and assess what forms of molecules can be attached to the pre-protein as cargo. A maj...
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Pre-queuosine or queuine (preQ1) is a guanine derivative that is involved in the biosynthetic pathway of the hypermodified tRNA nucleoside queuosine (Que). The core structure of preQ1 is represented by 7-(aminomethyl)-7-deazaguanine (preQ1 base). Here, we report the synthesis of three preQ1 base derivatives with complementary (15)N-labeling patterns, utilizing [(15)N]-KCN, [(15)N]-phthalimide, ...
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ژورنال
عنوان ژورنال: Interface Focus
سال: 2017
ISSN: 2042-8898,2042-8901
DOI: 10.1098/rsfs.2016.0117